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KMID : 1149220040120010195
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Korean Journal of Oriental Medical Prescription
2004 Volume.12 No. 1 p.195 ~ p.208
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Effects of Aconiti Tuber on the Change of Interleukin-6 and TNF-α Level induced by LPS I.C.V. Injection in Mice
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Koh Dong-Kyun
Yun Jeong-Moon
Lee Tae-Hee
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Abstract
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Objective: This study was conducted to investigate the effects of Aconiti Tuber on the plasma IL-6 and TNF-α level in mice stimulated by intracerebroventricular(I.C.V.) Injection of Lipopolysaccharide(LPS).
Method: 6 mice were assigned to each of the normal group, the control group, and the experimental group. In the normal group only saline was administered intragastrically, and in the control group LPS was injected intracerebro-ventricularly 1 hr after intragastric administration of saline. In the experimental groups (Aconiti Tuber 0.5g/kg, Aconiti Tuber 1.0g/kg, Aconiti Tuber 3.0g/kg) each sample was administered intragastrically to mice 1 hr prior to the stimulation by LPS I.C.V. Injection. To measure the plasma IL-6 and TNF-α level of mice, their blood samples were collected from retro-orbital plexus, immediately centrifuged at 4℃, and plasma was removed and stored frozen at -83℃ for later determination of plasma IL-6 and TNF-α.
Result : 1. LPS I.C.V. Injection increased plasma IL-6 level significantly in a dose-dependent manner compared with normal group(P<0.01). The plasma IL-6 concentration reached a significant maximal level about 1 hr after LPS I.C.V. Injection(P<0.001). LPS I.C.V. Injection increased plasma TNF-α level significantly in a dose-dependent manner(P<0.05). The plasma TNF-α concentration reached a significant maximal level about 1 hr after LPS I.C.V. Injection(P<0.001). 2. Sample A group to which Aconiti Tuber(0.5g/kg) was administered intragastrically 1 hr prior to the stimulation by LPS I.C.V. Injection showed insignificant lower plasma IL-6 level in 1 hr than control group(P<0.0635), and sample B group (Aconiti Tuber 1.0g/kg) showed significant lower plasma IL-6 level in 1 hr than control group(P〈0.05), and sample C group (Aconiti Tuber 3.0g/kg) showed significant lower IL-6 plasma level in 1 hr than control group(P<0.01). 3. sample A group to which Aconiti Tuber(0.5g/kg) was administered intragastrically 1 hr prior to the stimulation by LPS I.C.V. Injection showed insignificant lower plasma TNF-α level in 1 hr than control group(P>0.05), and Both sample B(1.0g/kg) and sample C(3.0g/kg) groups showed significant lower TNF-α plasma level in 1 hr than control group(P<0.01). These data revealed that Aconiti Tuber might have the anti inflammatory effect by reducing the plasma IL-6 and TNF-α level in a dose dependent manner in mice LPS I.C.V. Injection.
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KEYWORD
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Aconiti Tuber, LPS ICV, plasma IL-6 and TNF-α
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